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Alix Ventures

San Francisco, California · Invests in US · Website

Check size

$100K-$500K

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Thesis

Life sciences is experiencing unprecedented innovation through TechBio—companies that merge technology and biology. We partner with passionate, accomplished founders with unique insights to solve daring life science problems and significantly impact patient care. We provide capital, operational support, scientific guidance, and industry relationships to build transformative companies.

Likely not a fit for

Not interested in internationally located companies (outside USA). Avoid single indication diagnostics, single asset therapeutics, medical devices, microbiome, longevity/wellness focus, or non-life-sciences biology. Focus exclusively on TechBio driving patient impact.

How they invest

Lead or follow
Leads or follows
Typical decision
~2 weeks
Warm intro
Not required
Involvement
Advisor
Fund status
Actively deploying
Decision process
Partnership vote

Partners & team

6 contacts on file

CP

Chas Pulido

Founder & General Partner · Primary contact

NG

Nick Goldner

Venture Partner

MP

Mark Pulido

Operating Partner

BF

Brian Fiske

SAB Member - Drug Development

DD

Daniel Delubac

SAB Member - Diagnostics/Lab Services

JB

Jake Becraft

SAB Member - Syn Bio / CGT

Portfolio

XilisBiotech Life Sciences

Xilis is an oncology and precision health company focused on cancer diagnosis and treatment using micro-organoids. It develops ex-vivo technologies to accelerate drug discovery and aims to de-risk drug development and guide precision medicine.

ElegenBiotech Life Sciences

Elegen specializes in cell-free clonal gene synthesis through its ENFINIA DNA technology, providing innovative solutions for genetic engineering and synthetic biology.

64xEnterprise Software

64x.com offers digital imaging, web hosting, and design services, including posters, banners, and promotional product imprinting.

MythicRobotics Automation

Mythic is a pioneering AI processor company specializing in analog compute-in-memory technology, which delivers significant improvements in energy efficiency and performance for edge AI applications. Their Analog Matrix Processors enable high-performance computing for various sectors, including automotive, robotics, and defense, while drastically reducing power consumption and costs.

Endpoint HealthHealthcare Digital

Endpoint Health is a company valued at $150M or more, named in Y Combinator's Top Companies list, and launched to assist hospitals in treating critically ill patients.

ZafrensBiotech Life Sciences

Zafrens is pioneering a comprehensive experimental platform that integrates multi-omics and large perturbation libraries to uncover causal relationships in biology. Their technology, Z-Screen, allows for high-throughput experimentation with single-cell resolution, enabling simultaneous phenotypic and molecular screening for drug discovery.

TeikoBiotech Life Sciences

Teiko simplifies cytometry for global clinical trials with innovative products like blood preservation kits and multiparameter flow cytometry services.

CircularisFood Beverage

Circularis is focused on the cultivation of micro-algae to produce nutritious food and feed ingredients, emphasizing a circular bio-economy and sustainable practices.

GalateaBiotech Life Sciences

Galatea Bio is a biotechnology company focused on leveraging genetic diversity and advanced data analytics to drive innovations in healthcare. They provide clinical testing, genomic services, and biopharma R&D, while emphasizing the importance of underrepresented populations in genetic research.

Basil SystemsEnterprise Software

Basil Systems, also known as basil.bio, focuses on applied AI solutions.

Clora
Pair TeamHealthcare Digital

Pair Team is a connected care ecosystem that provides whole-person care by addressing medical, behavioral, and social needs through partnerships with local organizations and AI-enabled technology.

Recent activity

Alix Ventures in the news

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Research briefRead more
Alix Ventures Research Overview Alix Ventures is a thesis-driven, early-stage venture capital fund focused exclusively on TechBio—companies that merge technology and biology to drive transformative patient impact. Founded by healthcare entrepreneurs and venture capitalists with deep operational experience, Alix combines capital deployment with an extensive support ecosystem including a 1000+ member BIOS community, scientific advisory board of PhD founders, and operational expertise from veteran healthcare executives. Investment Thesis & Philosophy Core Thesis: Life sciences is experiencing unprecedented innovation through the rise of TechBio—companies that engineer biology using computational tools, automation, and computational infrastructure. This convergence is generating some of the most impactful healthcare solutions in decades. Alix believes the best opportunities lie in supporting passionate, accomplished founders with unique insights to solve daring problems in life sciences and significantly impact patient care. Mission Statement: "Driving Patient Impact" through partnerships with visionary founders building transformative biotechnology companies that advance human health. Unique Positioning: Unlike traditional biotech VCs, Alix operates as a venture studio ecosystem. The fund doesn't just provide capital—it actively builds a community, offers operational support, facilitates industry relationships, and leverages a scientific advisory board of bioengineering PhD founders to guide portfolio companies through technical challenges. Investment Focus & Sectors Alix Ventures maintains a focused mandate on TechBio and life sciences infrastructure across several core domains: Primary Focus Areas: Drug Development Infrastructure : Platforms enabling faster, more efficient drug discovery and development (e.g., synthesis platforms, manufacturing optimization) Tech-Enabled Therapeutics Platforms : Companies applying engineering biology and computation to develop novel treatments Tech-Enabled Diagnostics Platforms : Diagnostic tools leveraging AI, computation, and biotechnology Genomic Data Platforms : Infrastructure for genomic analysis, interpretation, and data management Gene Editing & Synthetic Biology : CRISPR alternatives, gene delivery, gene therapy platforms, strand therapeutics Clinical Trial Platforms : Technology enabling faster, more efficient clinical trials Lab Automation & Robotics : Automation infrastructure for life science research and manufacturing Cell & Gene Therapy Manufacturing : Infrastructure and manufacturing platforms for CGT and cell therapy Single-Cell Screening & Analysis : Technologies enabling high-throughput screening and profiling Current Portfolio Focus (18+ investments across stages): Xilis (precision medicine via patient-derived micro-organoids) Elegen (full-stack DNA writing) 64x (viral vector manufacturing) Mythic (therapeutic potency enhancement) Endpoint Health (precision immunology) Zafrens (single-cell screening) Teiko (immune system profiling) Strand (logic-gated gene therapy) Exthymic (cell therapy manufacturing) STRM (non-viral gene delivery) Chemify (chemical synthesis automation) Cytosolix (tumor acidity targeting) Stage Focus & Check Size Stage Preference: Primary : Pre-seed and Seed ($250K-$500K initial tickets) Secondary : Series A (selective follow-ons and co-investments) Venture Studio Model : Incubates companies from concept Check Size: Current range: $100K - $500K (per API data showing min=$100K, max=$500K) Typical deployment: $250K-$350K for seed stage companies Active follow-on reserves for portfolio companies Mark Pulido (Operating Partner, 5x CEO) provides strategic operational support Portfolio Performance: 700+ jobs created across portfolio $850M+ total capital raised by portfolio companies $2.5B+ collective value created 25% female-founded companies 40% diversity representation 60% solo-founded companies Lead Tendency & Decision Process Lead Tendency: Both (Leads and Co-Leads) Alix actively leads seed rounds while also participating in syndicated rounds with complementary co-investors. The firm's strong operational support and scientific expertise make them attractive co-investors for larger rounds. Decision Process: Partnership Model The investment team is structured around partnerships and scientific expertise: Chas Pulido - Founder & General Partner (3rd generation healthcare CEO, serial entrepreneur, venture capitalist) Nick Goldner - Venture Partner (CEO Founder, WashU PhD scientist) Mark Pulido - Operating Partner (5x CEO with deep healthcare industry experience) Scientific Advisory Board - Brian Fiske (Mythic co-founder, MIT PhD), Daniel Delubac (Startup exec at Counsyl/Guardant), Jake Becraft (Strand Therapeutics co-founder/CEO, MIT PhD) Decision Timeline: 2-3 weeks (typical for seed-stage VCs) The partnership model and deep scientific expertise enable quick evaluation of technical feasibility and commercial potential. Recent Activity & Fund Status Fund Status: Actively Deploying Alix continues consistent deployment from current fund with recent activities spanning 2025-2026. Recent Notable Activity: October 2025: Investment in iOrganBio (organoid technology) Ongoing deployment across pre-seed and seed stages Strong follow-on support for portfolio companies advancing to Series A Active ecosystem building through BIOS community (1000+ members) Portfolio Milestone Companies: Endpoint Health - Secured oversubscribed $52M Series A with active support from Alix Zafrens - Co-founder Kayj Shannon now part of Alix's Venture Residency program Multiple exits - Circularis acquired by Gingko Bioworks Investment Team & Leadership Chas Pulido, Founder & General Partner 3rd generation healthcare CEO with serial entrepreneurship background Venture capital experience building the fund Mission-driven focus on patient impact Sourcing and deal evaluation Nick Goldner, Venture Partner CEO Founder background WashU PhD scientist (deep technical expertise) Building TechBio future Scientific evaluation and due diligence Mark Pulido, Operating Partner 5-time CEO with 40+ years of leadership experience Healthcare IT, Health Services, Big Pharma, Diagnostics background Private Equity and Venture-backed startup experience Direct portfolio and fund support Scientific Advisory Board: Brian Fiske (Co-Founder/CSO Mythic, MIT PhD) - Drug Development expertise Daniel Delubac (Startup exec Counsyl/Guardant/Freenome/Xilis, CMU PhD) - Diagnostics/Lab Services Jake Becraft (Co-Founder/CEO Strand Therapeutics, MIT PhD) - Synthetic Biology/Cell & Gene Therapy Founder Preferences Alix seeks founders who embody: Visionary & Passionate : "Passionate accomplished founders with unique insights" - driven to solve daring life science problems Technical Expertise : PhDs, bioengineers, MDs with deep domain knowledge Operational Capability : Demonstrated execution ability; preference for founders with prior startup or industry experience Founder Mentality : Understanding of the "this needs to be done yesterday" urgency in healthcare Coachability : Openness to partnership, operational guidance, and strategic input Diversity : Active recruitment of diverse founders (40% diversity representation in portfolio) Summary Alix Ventures is a specialized, thesis-driven early-stage VC fund focused exclusively on TechBio. Founded by healthcare entrepreneurs with deep operational experience, the firm combines disciplined capital deployment ($250K-$500K typical checks) with an extensive ecosystem of scientific advisory, operational support, and industry relationships. The 18+ portfolio companies (700+ jobs created, $2.5B+ value) reflect a focus on transformative technologies across drug development infrastructure, diagnostics, gene therapy, and cell manufacturing. With active portfolio support evidenced by milestone exits (Endpoint Health's $52M Series A) and visible co-investor relationships, Alix Ventures has established itself as the premier early-stage TechBio partner for passionate founders seeking both capital and operational partnership to build world-changing companies.

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